Device
Part:BBa_K392042:Design
Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi Group: iGEM10_Osaka (2010-10-25)
exoglucanase/CBM secretion testing construct for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 821
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 280
Illegal NgoMIV site found at 454
Illegal NgoMIV site found at 827
Illegal NgoMIV site found at 1329 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 874
Illegal SapI.rc site found at 957
Design Notes
Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.
Source
Assembly of R0010, B0034, J32015, K392007, K392014, B0010.