Device

Part:BBa_K392042:Design

Designed by: Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi   Group: iGEM10_Osaka   (2010-10-25)


exoglucanase/CBM secretion testing construct for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 821
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 827
    Illegal NgoMIV site found at 1329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 874
    Illegal SapI.rc site found at 957


Design Notes

Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.


Source

Assembly of R0010, B0034, J32015, K392007, K392014, B0010.

References